They can, provided the whole chain is documented: how the land was acquired, that it was lawfully owned, the terms of the sale, the tax treatment, and the movement of the proceeds. The sale itself is one link, not the answer.
Prove the ownership before the sale
The weakest point in most property-based cases is the beginning rather than the end. A closing statement from last year is easy to produce; evidence of how the parcel came into the family thirty years ago may not be. Start at the registry and work forward, collecting the instrument that transferred the land, any mortgage and its discharge, assessment records, and the tax filings that reported the eventual gain. Where the land was inherited, the estate documents become part of the chain. Where it was bought, the source of that earlier purchase money may itself need explaining, and how far back this reaches is a judgement to take with an adviser rather than to guess. Present the chain as a numbered sequence with a document behind each step, so a reviewer can follow it without reconstructing it.
Hypothetical example: A civil engineer plans to invest proceeds from exercised stock options and a sale of privately held shares. The first review should produce a source-of-funds chain from the option grant through exercise, tax treatment, sale, bank deposits, and transfer to the commercial enterprise. Brokerage statements, tax records, share-sale documents, and wire confirmations settle different links. EB-5 capital must be lawfully obtained and placed at commercial risk; the project must result in the required ten full-time jobs for qualifying American workers. The investor must also hold a qualifying management or policy-formulation role. Petition approval begins a later immigration process and does not itself confer residence.